expresslink™ t4 dna ligase catalyzes the ligation of blunt- or cohesive-end dna fragments in only 5 minutes at room temperature (25�� c), and pcr fragments with ��a" overhangs
t4 dna ligase catalyzes the formation of phosphodiester bonds in the presence of atp between double-stranded dnas with 3�� hydroxyl and 5�� phosphate termini. the unique t4 dna ligase buffer optimizes l
iptg (isopropylthio-��-galactoside) is an inducer of ��-galactosidase activity in bacteria and is suitable for use with x-gal or bluo-gal to detect lac gene activity in cloning procedures.
zero blunt® topo® pcr cloning kit provides a highly efficient, 5-minute, one-step cloning strategy ("topo® cloning") for the direct insertion of blunt-ended pcr prod
the zero blunt® pcr cloning kit offers an easy method for high-efficiency (>80%) cloning of blunt-end pcr products amplified with proof-reading, thermostable dna polymerases.
the ta cloning® kit with pcr™2.1 vector provides a quick, one-step cloning strategy for directly inserting a taq-amplified pcr product into a plasmid vector. the ta clon
the topo® ta cloning® kits for sequencing provide a highly efficient, 5-minute, one-step cloning strategy ("topo® cloning") for the direct insertion of taq polymeras
topo® ta cloning® kits for subcloning provide a highly efficient, 5-minute, one-step cloning strategy ("topo® cloning") for the direct insertion of taq polymerase�Cam
the nativepage™ novex® bis-tris gel system is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analysis of native membr
novex® tricine gels are useful for resolving low molecular weight proteins and peptides. in the tricine system developed by schaegger and von jagow (1), tricine replaces glyci
the silverquest™ silver staining kit provides a rapid and easy method to silver stain proteins and dna in polyacrylamide gels. silver staining allows detection of most protei